Peptide Applications Support

Peptides can be used in a wide variety of research applications:

Immunology

• Immune Monitoring

  The tracking of immune responses after a particular drug treatment or immunization is a useful way of monitoring the effectiveness of treatments.  There are numerous methods used for the purposes of monitoring immune responses.

  • ELISPOT

  • Intracellular Cytokine Staining

  • Proliferation Assays

    • CFSE

    • ³H Thymidine

• Epitope Discovery

  The aim of epitope mapping projects is to identify and characterize novel epitopes from a protein that are recognized by the immune system.   By identifying novel epitopes, strategies can be developed to produce immunotherapies or vaccines for a wide range of disease areas such as cancers or infectious diseases.

  • T cell Epitope Discovery

    • ELISPOT

    • Intracellular Cytokine Staining

    • Proliferation Assays

    • MHC Peptide Binding Assays

  • B Cell Epitope Mapping

    • ELISA

    • Inhibition Assays

Cell Signalling

The phosphorylation/dephosphorylation process is regulated by kinases and phosphatases respectively. Many hundreds of these individual enzymes have been identified and disruption to any one of them can have multiple effects on downstream pathways. A variety of disease pathologies, particularly cancer, have been associated with mutations or deletions in signalling pathways, making the identification of new kinases and phosphatases, and also their inhibitors, an important target for many research projects.

• Kinase Assays

  • Radio-labeled Kinase Assay

  • ELISA Based Assay

  • Fluorescent Based Assay

    • Fluorescence Polarization Assay

    • Fluorescence Resonance Energy Transfer (FRET)

    • Time Resolved Fluorescence (TRF)

  • Mobility Shift Assays

thinkpeptides is a brand of ProImmune